Interpretes Principales: Lisa Edelstein (Dr. Lisa Cuddy), Omar Epps (Dr. Eric Foreman), Hugh Laurie (Dr. Gregory House), Robert Sean Leonard (Dr. James Wilson), Jesse Spencer (Dr. Robert Chase), Jennifer Morrison (Dr. Allison Cameron).
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Otros Artistas: Chi McBride (Edward Vogler), Kenneth Choi (Dr. Lim), Michael Goorjian (Sean Randolph), Marin Hinkle (Naomi Randolph), Reggie Jordan (Anestesista), John Berg (Dr. Prather), S.E. Perry (Oficial Davis), Guy Camilleri (Heyden Brown), Kevin Brief (Oficial Angle), Diane Sellers (Gail Friedman), Veronica Brown (Mujer del Consejo), Michael Simpson (Andrew Kaplan), Natalie Shaw (Rachel Kaplan), Ron Perkins (Dr. Simpson).
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